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Quantification of regulatory T cells in patients with systemic lupus erythematosus
Ist Teil von
Journal of autoimmunity, 2003-11, Vol.21 (3), p.273-276
Ort / Verlag
England: Elsevier Ltd
Erscheinungsjahr
2003
Quelle
MEDLINE
Beschreibungen/Notizen
CD4
+T cells that constitutively express CD25 exhibit powerful suppressive properties. Such cells have been denominated regulatory T cells (T
R). Alterations in T
Rcells are known to cause organ-specific autoimmune disease in animal models. The aim of this work was to quantify CD4
+CD25
+T cells in patients with systemic lupus erythematosus (SLE). Thirty untreated patients (ten with active disease) and ten healthy volunteers were studied. Flow cytometry was used to quantify cell populations. CD4
+CD69
+, CD4
+CD25
+and CD4
+CD25
brightcells were considered. Peripheral blood mononuclear cell cultures were performed and supernatants collected for IL-10 and 12 measurement. CD4
+CD25
+cells were significantly decreased in patients with active disease when compared to control subjects and patients without disease activity (
P<0.001). CD4
+CD69
+cells were increased in patients with active disease when compared to controls (
P=0.041). Accordingly, CD4
+CD25
brightcells were decreased in patients with active disease compared to healthy subjects (
P<0.001). IL-12 production was hampered in cells from patients during periods of active disease when compared to healthy controls and patients during remission (
P<0.001). We observed a correlation between decreased T
Rnumber and reduced IL-12 mononuclear cell production (
r=0.362,
P=0.05). This work demonstrates that CD4
+CD25
+T cells are decreased in patients with clinically active SLE.