Details

Autor(en) / Beteiligte

• Wei, Hui-Yong
• Jiang, Li-Fang
• Fang, Dan-Yun
• Guo, Hui-Yu

Titel

Dengue virus type 2 infects human endothelial cells through binding of the viral envelope glycoprotein to cell surface polypeptides

Ist Teil von

• Journal of general virology, 2003-11, Vol.84 (11), p.3095-3098

Ort / Verlag

England: Soc General Microbiol

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, People's Republic of China 2 Medical Laboratory Center, Guangdong Provincial People's Hospital, 106 Zhongshan Road II, Guangzhou 510080, People's Republic of China Correspondence Hui-Yong Wei (at Zhongshan Medical College) hywei2002{at}yahoo.com.cn The endothelial cell line ECV304, derived from human umbilical cord and identified to be susceptible to dengue virus type 2 (DEN-2) infection, was used to study the molecular mechanism of DEN-2 binding to endothelial cells. DEN-2 was found by virus overlay protein-binding assays (VOPBAs) to bind to three ECV304 cell membrane proteins with molecular masses of 29, 34 and 43 kDa. Only a single protein of 29 kDa was observed when VOPBAs were carried out using preparations of trypsin-treated ECV304 cells. Pre-incubation of live ECV304 cells in culture or cell membrane proteins in modified VOPBAs with the recombinant DEN-2 envelope glycoprotein (rEgp) inhibited DEN-2 infection and blocked virus binding to the three proteins identified. These results indicate that DEN-2 rEgp could bind to three proteins on the surface of ECV304 cells. This virus–cell interaction may be associated with the receptor complex specific for DEN-2 infection of endothelial cells.