Details

Autor(en) / Beteiligte

• Tacket, Carol O.
• Mason, Hugh S.
• Losonsky, Genevieve
• Estes, Mary K.
• Levine, Myron M.
• Arntzen, Charles J.

Titel

Human Immune Responses to a Novel Norwalk Virus Vaccine Delivered in Transgenic Potatoes

Ist Teil von

• The Journal of infectious diseases, 2000-07, Vol.182 (1), p.302-305

Ort / Verlag

Chicago, IL: The University of Chicago Press

Erscheinungsjahr

2000

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• A new approach for delivering vaccine antigens is the use of inexpensive, plentiful, plantbased oral vaccines. Norwalk virus capsid protein (NVCP), assembled into virus-like particles, was used as a test antigen, to determine whether immune responses could be generated in volunteers who ingested transgenic potatoes. Twenty-four healthy adult volunteers received 2 or 3 doses of transgenic potato (n = 20) or 3 doses of wild-type potato (n = 4). Each dose consisted of 150 g of raw, peeled, diced potato that contained 215–751 μg of NVCP. Nineteen (95%) of 20 volunteers who ingested transgenic potatoes developed significant increases in the numbers of specific IgA antibody-secreting cells. Four (20%) of 20 volunteers developed specific serum IgG, and 6 (30%) of 20 volunteers developed specific stool IgA. Overall, 19 of 20 volunteers developed an immune response of some kind, although the level of serum antibody increases was modest.