Biochemical and biophysical research communications, 2000-07, Vol.273 (3), p.859-864
2000
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- Autor(en) / Beteiligte
- Titel
- Glucose Deprivation Does Not Affect GLUT1 Targeting in 3T3-L1 Adipocytes
- Ist Teil von
- Biochemical and biophysical research communications, 2000-07, Vol.273 (3), p.859-864
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2000
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- We have previously demonstrated that glucose deprivation alters the glycosylation of the GLUT1 glucose transporter in 3T3-L1 adipocytes. Many aberrantly glycosylated proteins are retained in the endoplasmic reticulum by interaction with chaperones. Herein, we use three independent procedures to show that GLUT1 is targeted to the plasma membrane, despite alterations in glycosylation. While earlier experiments revealed that plasma membrane targeting of aglyco GLUT 1 transporter was significantly reduced, our data show for the first time that altered glycosylation provides sufficient information to drive appropriate trafficking.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-291XeISSN: 1090-2104DOI: 10.1006/bbrc.2000.2985Titel-ID: cdi_proquest_miscellaneous_71226411
- Format
- –
- Schlagworte
- 3T3 Cells, 3T3-L1 adipocytes, Adipocytes - drug effects, Adipocytes - metabolism, Animals, Biotin - metabolism, biotinylation, Cell Fractionation, Cell Membrane - metabolism, Cell Membrane - ultrastructure, Glucose - pharmacology, Glucose Transporter Type 1, GLUT1, Glycosylation, Mice, Monosaccharide Transport Proteins - metabolism, plasma membrane fragments, subcellular fractionation, targeting