Details

Autor(en) / Beteiligte

• Ohta, Mitsuhiro
• Mizuta, Ikuko
• Ohta, Kiyoe
• Nishimura, Masataka
• Mizuta, Eiji
• Hayashi, Kyozo
• Kuno, Sadako

Titel

Apomorphine Up-Regulates NGF and GDNF Synthesis in Cultured Mouse Astrocytes

Ist Teil von

• Biochemical and biophysical research communications, 2000-05, Vol.272 (1), p.18-22

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2000

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Apomorphine, a D1/D2 dopamine agonist, is an antiparkinsonian drug. We examined the effects of apomorphine on synthesis of neurotrophic factors in cultured mouse astrocytes. After 24 h incubation with apomorphine, NGF and GDNF contents in the culture medium increased to 122-fold and 1.8-fold of the control, respectively; whereas the BDNF content did not change significantly. In Northern blot analysis, expression of NGF mRNA in astrocytes reached the maximum level at 6 h after addition of the drug. By semiquantitative RT-PCR analysis, the GDNF transcript level was found to reach 2.9-fold of the control level at 15 h. These results suggest that apomorphine may exert neuroprotective effects by stimulation of NGF and GDNF synthesis in astrocytes.