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Apomorphine Up-Regulates NGF and GDNF Synthesis in Cultured Mouse Astrocytes
Ist Teil von
Biochemical and biophysical research communications, 2000-05, Vol.272 (1), p.18-22
Ort / Verlag
United States: Elsevier Inc
Erscheinungsjahr
2000
Quelle
Elsevier ScienceDirect Journals
Beschreibungen/Notizen
Apomorphine, a D1/D2 dopamine agonist, is an antiparkinsonian drug. We examined the effects of apomorphine on synthesis of neurotrophic factors in cultured mouse astrocytes. After 24 h incubation with apomorphine, NGF and GDNF contents in the culture medium increased to 122-fold and 1.8-fold of the control, respectively; whereas the BDNF content did not change significantly. In Northern blot analysis, expression of NGF mRNA in astrocytes reached the maximum level at 6 h after addition of the drug. By semiquantitative RT-PCR analysis, the GDNF transcript level was found to reach 2.9-fold of the control level at 15 h. These results suggest that apomorphine may exert neuroprotective effects by stimulation of NGF and GDNF synthesis in astrocytes.