Details

Autor(en) / Beteiligte

• Wilcoxen, Scott C
• Kirkman, Erlinda
• Dowdell, Kennichi C
• Stohlman, Stephen A

Titel

Gender-Dependent IL-12 Secretion by APC Is Regulated by IL-10

Ist Teil von

• The Journal of immunology (1950), 2000-06, Vol.164 (12), p.6237-6243

Ort / Verlag

United States: Am Assoc Immnol

Erscheinungsjahr

2000

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Female SJL mice preferentially mount Th1-immune responses and are susceptible to the active induction of experimental allergic encephalomyelitis. By contrast, young adult male SJL are resistant to experimental allergic encephalomyelitis due to an APC-dependent induction of Th2 cells. The basis for this gender-dependent differential T cell induction was examined by analysis of macrophage APC cytokine secretion during T cell activation. APC derived from females secrete IL-12, but not IL-10, during T cell activation. By contrast, APC derived from males secrete IL-10, but not IL-12, during T cell activation. Activation of T cells with APC derived from the opposite sex demonstrated that these cytokines were derived from the respective APC populations. Furthermore, inhibition of IL-10, but not TGF-beta, during T cell activation resulted in the secretion of IL-12 by male-derived APC. APC from naive male mice, in which IL-10 was reduced in vivo before isolation, also secrete IL-12, demonstrating altered APC cytokine secretion was due to an environment high in IL-10 before Ag encounter. Finally, APC derived from castrated male mice preferentially secrete IL-12 during T cell activation. These data demonstrate a link between gonadal hormones and APC activity and suggest that these hormones alter the APC, thereby influencing cytokine secretion during initial T cell activation.