Oncogene, 2001-09, Vol.20 (39), p.5554-5561
2001
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- Autor(en) / Beteiligte
- Titel
- Conserved synteny between the Fugu and human PTEN locus and the evolutionary conservation of vertebrate PTEN function
- Ist Teil von
- Oncogene, 2001-09, Vol.20 (39), p.5554-5561
- Ort / Verlag
- Basingstoke: Nature Publishing
- Erscheinungsjahr
- 2001
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Mutations of PTEN, which encodes a protein-tyrosine and lipid phosphatase, are prevalent in a variety of human cancers. The human genome 'draft' sequence still lacks organization and much of the PTEN and adjacent loci remain undefined. The pufferfish, Fugu rubripes, by virtue of having a compact genome represents an excellent template for rapid vertebrate gene discovery. Sequencing of 56 kb from the Fugu pten (fpten) locus identified four complete genes and one partial gene homologous to human genes. Genes neighboring fpten include a PAPS synthase (fpapss2) differentially expressed between non-metastatic/metastatic human carcinoma cell lines, an inositol phosphatase (fminpp1) and an omega class glutathione-S-transferase (fgsto). We have determined the order of human BAC clones at the hPTEN locus and that the locus contains hPAPSS2 and hMINPP1 genes oriented as are their Fugu orthologs. Although the human genes span 500 kb, the Fugu genes lie within only 22 kb due to the compressed intronic and intergenic regions that typify this genome. Interestingly, and providing striking evidence of regulatory element conservation between widely divergent vertebrate species, the compact 2.1 kb fpten promoter is active in human cells. Also, like hPTEN, fpten has a growth and tumor suppressor activity in human glioblastoma cells, demonstrating conservation of protein function.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0950-9232eISSN: 1476-5594DOI: 10.1038/sj.onc.1204679Titel-ID: cdi_proquest_miscellaneous_71196428
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Biological and medical sciences, Cell Division, Cell growth, Cell physiology, Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes, Cloning, Molecular, Conserved Sequence, Divergence, Evolution, Molecular, Evolutionary conservation, Fish Proteins, Fishes - genetics, Fishes - metabolism, fpapss2 gene, fpten gene, Fugu, Fugu rubripes, Fundamental and applied biological sciences. Psychology, Genes, Genes, Tumor Suppressor, Genetic Linkage, Genomes, Glioblastoma, Glioblastoma - pathology, Glioblastoma cells, Glutathione, Glutathione transferase, Glutathione Transferase - genetics, hMINPP1 gene, hPAPSS2 gene, Humans, Inositol, Kinases, Metastases, Metastasis, Molecular and cellular biology, Molecular Sequence Data, Multienzyme Complexes - genetics, Mutation, myo-Inositol-1 (or 4)-monophosphatase, Nucleotide sequence, Phosphatase, Phosphoric Monoester Hydrolases - genetics, Phosphoric Monoester Hydrolases - physiology, Promoter Regions, Genetic, Prostate cancer, Protein Tyrosine Phosphatases - genetics, Protein Tyrosine Phosphatases - physiology, Protein-tyrosine-phosphatase, Proteins, PTEN gene, PTEN Phosphohydrolase, PTEN protein, RNA, Messenger - biosynthesis, Sequence Homology, Amino Acid, Sulfate Adenylyltransferase - genetics, Synteny, Tumor cell lines, Tumor Cells, Cultured, Tumor suppressor genes, Tumor Suppressor Proteins, Tumors, Womens health