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Immunostaining of ganglioside GD1b, GD3 and GM1 in rat cerebellum: Cellular layer and cell type specific associations
Journal of neuroscience research, 2000-05, Vol.60 (4), p.531-542
Molander, Marie
Berthold, Claes-Henric
Persson, Håkan
Fredman, Pam
2000
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Molander, Marie
Berthold, Claes-Henric
Persson, Håkan
Fredman, Pam
Titel
Immunostaining of ganglioside GD1b, GD3 and GM1 in rat cerebellum: Cellular layer and cell type specific associations
Ist Teil von
Journal of neuroscience research, 2000-05, Vol.60 (4), p.531-542
Ort / Verlag
New York: John Wiley & Sons, Inc
Erscheinungsjahr
2000
Quelle
MEDLINE
Beschreibungen/Notizen
We have studied the cellular distribution of gangliosides GD1b, GD3 and GM1 in rat cerebellum by immunostaining, using monoclonal antibodies and confocal microscopy. Antibodies against astroglial, neuronal and synaptic vesicle associated molecules were used for colocalization analyses. In the gray matter, the anti‐GD1b antibody stained thin strands in the molecular layer (ML), interpreted as Bergman glia fibers based on colocalized staining with anti‐glial fibrillary acidic protein (GFAP). The neuropil in the granule (GL) and Purkinje (PL) cell layers was also anti‐GD1b positive. The anti‐GD3 antibody stained the ML, the neuropil in the GL and PL and also the granule and Purkinje cell bodies, appearing intracytoplasmically and vesicle associated. Anti‐GD1b and anti‐GD3 staining in the GL glomeruli were colocalized with anti‐synaptophysin staining. The anti‐GM1 antibody stained cell bodies in the ML but they could not be characterized in colocalization experiments. The GL and PL were not stained with the anti‐GM1 antibody. In the white matter, different staining patterns were seen for the gangliosides, the anti‐GM1 staining being the most intense. This study shows cellular layer and cell type specific associations of the investigated gangliosides and localization of GD1b and GD3 at synaptic sites, warranting further studies on their role in synaptic mechanisms. J. Neurosci. Res. 60:531–542, 2000 © 2000 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0360-4012
eISSN: 1097-4547
DOI: 10.1002/(SICI)1097-4547(20000515)60:4<531::AID-JNR12>3.0.CO;2-6
Titel-ID: cdi_proquest_miscellaneous_71094578
Format
–
Schlagworte
Animals
,
Astrocytes - metabolism
,
brain
,
Cerebellum - cytology
,
Cerebellum - metabolism
,
Fluorescent Antibody Technique, Indirect
,
G(M1) Ganglioside - metabolism
,
Gangliosides - metabolism
,
glycosphingolipids
,
Immunohistochemistry
,
Microscopy, Confocal
,
Nerve Fibers - metabolism
,
Neuropil - metabolism
,
Purkinje Cells - metabolism
,
Rats
,
Rats, Sprague-Dawley
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