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Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov
Ist Teil von
International journal of systematic and evolutionary microbiology, 2000-03, Vol.50 (2), p.901-907
EP Ivanova, LA Romanenko, J Chun, MH Matte, GR Matte, VV Mikhailov, VI Svetashev, A Huq, T Maugel and RR Colwell
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt St, Baltimore, MD 21202, USA
Two bacterial strains, KMM 227(T) and 231(T), were isolated from seawater
samples collected from the north-western Pacific Ocean at a depth of
4000--5000 m and were characterized using polyphasic taxonomy. Both were
Gram-negative, psychrotolerant, heterotrophic, aerobic and required NaCl
for growth (0.6--15.0%). The temperature for growth was 4--30 degrees C.
Both strains were rod-shaped, with a single flagellum. However, strain KMM
231(T) revealed a single long fimbrium. Cellular fatty acids detected in
the isolates were predominantly odd-numbered and iso-branched, with 15 and
17 carbons (ca. 70%). Also present were saturated and monounsaturated
straight-chain fatty acids. Results of phylogenetic analyses, employing
three tree-making methods, strongly indicated that the two strains formed a
distinct lineage within a clade containing the genera Alteromonas,
Colwellia and Pseudoalteromonas, in the gamma-Proteobacteria. The two
strains shared 16S rDNA sequence similarity of 96.9% and genomic DNA
relatedness of 27%; the latter was determined by dot-blot hybridization.
The strains were differentiated by the presence of fimbria, production of
chitinase, ability to grow on 15% NaCl and BIOLOG profiles. Given the
polyphasic evidence accumulated in this study, it is proposed that the two
deep-sea isolates be classified in the genus Idiomarina gen. nov., as
Idiomarina abyssalis sp. nov. (type strain is KMM 227(T)) and Idiomarina
zobellii sp. nov. (type strain is KMM 231(T)).