Details

Autor(en) / Beteiligte

• Frucht, David M
• Aringer, Martin
• Galon, Jerome
• Danning, Carol
• Brown, Martin
• Fan, Samuel
• Centola, Michael
• Wu, Chang-You
• Yamada, Nubuo
• El Gabalawy, Hani
• O'Shea, John J

Titel

Stat4 Is Expressed in Activated Peripheral Blood Monocytes, Dendritic Cells, and Macrophages at Sites of Th1-Mediated Inflammation

Ist Teil von

• The Journal of immunology (1950), 2000-05, Vol.164 (9), p.4659-4664

Ort / Verlag

United States: Am Assoc Immnol

Erscheinungsjahr

2000

Quelle

MEDLINE

Beschreibungen/Notizen

• Stat4 is a key transcription factor involved in promoting cell-mediated immunity, whose expression in mature cells has been reported to be restricted to T and NK cells. We demonstrate here, however, that Stat4 expression is not restricted to lymphoid cells. In their basal state, monocytes do not express Stat4. Upon activation, however, IFN-gamma- and LPS-treated monocytes and dendritic cells express high levels of Stat4. Monocyte-expressed Stat4 in humans is phosphorylated in response to IFN-alpha, but not IL-12. In contrast, the Th2 cytokines, IL-4 and IL-10, specifically down-regulate Stat4 expression in activated monocytes, while having little effect on Stat6 expression. Moreover, macrophages in synovial tissue obtained from patients with rheumatoid arthritis express Stat4 in vivo, suggesting a potential role in a prototypical Th1-mediated human disease. IFN-alpha-induced Stat4 activation in human monocytes represents a previously unrecognized signaling pathway at sites of Th1 inflammation.