Details

Autor(en) / Beteiligte

• Goes, Annette
• Wouters, Diana
• Pol, Susanne M. A.
• Huizinga, Ruth
• Ronken, Eric
• Adamson, Peter
• Greenwood, John
• Dijkstra, Christine D.
• Vries, Helga E.

Titel

Reactive oxygen species enhance the migration of monocytes across the blood‐brain barrier in vitro

Ist Teil von

• The FASEB journal, 2001-08, Vol.15 (10), p.1852-1854

Ort / Verlag

United States: Federation of American Societies for Experimental Biology

Erscheinungsjahr

2001

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• ABSTRACT Reactive oxygen species (ROS) are involved in the pathogenesis of several inflammatory and degenerative diseases, including multiple sclerosis (MS), an inflammatory disease of the central nervous system. We investigated the potential involvement of ROS in the interaction of monocytes with cerebral endothelium, because this is likely to be an early event in the development of MS lesions. ROS are produced via two main pathways, one involving NADPH oxidase complex and the other involving xanthine oxidase (XO). We examined the effects of ROS, ROS scavengers, and ROS inhibitors of both pathways on the migration of monocytes across the blood‐brain barrier in vitro. Scavengers and inhibitors of XO predominantly inhibited monocyte migration, whereas inhibitors and scavengers of the NADPH oxidase complex had no effect. Exposure of cerebral endothelial cells (CEC) to superoxide (O2−) resulted in enhanced migration and adhesion of monocytes as well as disruption of the tight junctions, whereas hydroxyl radicals and hydrogen peroxide induced no significant effect on these parameters. Underlying mechanisms of the observed changes were found to reside in the phospholipase C‐mediated signal transduction cascade, subsequent accumulation of inositol 1,4,5‐trisphosphate, and mobilization of intracellular calcium. We conclude that O2− is a signaling molecule that is produced during the firm adhesion of monocytes to CEC, which triggers cytoskeletal rearrangements allowing infiltration of monocytes into the brain.