Cell, 2000-03, Vol.100 (6), p.655-669
2000
Details
- Autor(en) / Beteiligte
- Titel
- A Novel Transcription Factor, T-bet, Directs Th1 Lineage Commitment
- Ist Teil von
- Cell, 2000-03, Vol.100 (6), p.655-669
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2000
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Naive T helper cells differentiate into two subsets, Th1 and Th2, each with distinct functions and cytokine profiles. Here, we report the isolation of T-bet, a Th1-specific T box transcription factor that controls the expression of the hallmark Th1 cytokine, IFNγ. T-bet expression correlates with IFNγ expression in Th1 and NK cells. Ectopic expression of T-bet both transactivates the IFNγ gene and induces endogenous IFNγ production. Remarkably, retroviral gene transduction of T-bet into polarized Th2 and Tc2 primary T cells redirects them into Th1 and Tc1 cells, respectively, as evidenced by the simultaneous induction of IFNγ and repression of IL-4 and IL-5. Thus, T-bet initiates Th1 lineage development from naive Thp cells both by activating Th1 genetic programs and by repressing the opposing Th2 programs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0092-8674eISSN: 1097-4172DOI: 10.1016/S0092-8674(00)80702-3Titel-ID: cdi_proquest_miscellaneous_71034867
- Format
- –
- Schlagworte
- AIDS/HIV, Amino Acid Sequence, Animals, B-Lymphocytes - physiology, Base Sequence, Cell Differentiation - immunology, Cell Lineage - physiology, Cell Polarity - immunology, Cloning, Molecular, Flow Cytometry, Gene Expression - immunology, Genes, Reporter, Green Fluorescent Proteins, Indicators and Reagents - metabolism, Interferon-gamma - genetics, Interleukin-4 - genetics, Interleukin-5 - genetics, Killer Cells, Natural - physiology, Luminescent Proteins - genetics, Mice, Mice, Inbred BALB C, Mice, Transgenic, Molecular Sequence Data, Signal Transduction - immunology, T-bet protein, T-Box Domain Proteins, Th1 Cells - chemistry, Th1 Cells - cytology, Th1 Cells - immunology, Th2 Cells - cytology, Th2 Cells - immunology, Transcription Factors - genetics, Transcription Factors - metabolism