Details

Autor(en) / Beteiligte

• Ebata, N.
• Sawa, Y.
• Nodasaka, Y.
• Yamaoka, Y.
• Yoshida, S.
• Totsuka, Y.

Titel

Immunoelectron microscopic study of PECAM-1 expression on lymphatic endothelium of the human tongue

Ist Teil von

• Tissue & cell, 2001-06, Vol.33 (3), p.211-218

Ort / Verlag

Scotland: Elsevier Ltd

Erscheinungsjahr

2001

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• The expression of platelet–endothelial cell adhesion molecule-1 (PECAM-1) on lymphatic and blood vessels of the human tongue was examined with fluorescence and transmission electron microscopy (TEM). The study used anti-desmoplakins antiserum for light microscopic identification of the lymphatic vessels, plus a pre-embedding immunogold electron microscopic technique for TEM observations. Before making TEM observations, cryostat serial sections were immunostained with anti-desmoplakins or anti-PECAM-1 and then embedded. Semithin sections from each cryostat section were photographed under a light microscope and compared in order to identify the lymphatic vessels expressing PECAM-1. In fluorescence microscopy, PECAM-1 expression on lymphatic vessels was weaker than that on blood vessels. TEM observations showed that PECAM-1 expression on the blood vessels was observed only on the luminal surface of the endothelium. In lymphatic vessels, PECAM-1 expression was found both on the luminal and abluminal surfaces of the endothelium. The density ofthe PECAM-1 reaction products was lower in lymphatic vessels than in blood vessels. The density of PECAM-1 reaction products on the luminal surface of lymphatic vessels was higher than on the abluminal surfaces. The results suggest that blood vessels are more active than lymphatic vessels in leukocyte migration. The expression of PECAM-1 on the abluminal surface of lymphatic endothelium may allow leukocytes to adhere to the endothelium and interact in their migration from tissue into lymphatic vessels.