Details

Autor(en) / Beteiligte

• McKerrow, J.
• Vagg, S.
• McKinney, T.
• Seviour, E.M.
• Maszenan, A.M.
• Brooks, P.
• Seviour, R.J.

Titel

A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram‐positive bacteria

Ist Teil von

• Letters in applied microbiology, 2000-03, Vol.30 (3), p.178-182

Ort / Verlag

Oxford, UK: Blackwell Science Ltd

Erscheinungsjahr

2000

Quelle

Wiley

Beschreibungen/Notizen

• A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram‐positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o‐phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram‐positive bacteria is described, where 10 mg or less of dry cells is required.