Details

Autor(en) / Beteiligte

• Li, Jun
• Chen, Fabian
• Epstein, Jonathan A.

Titel

Neural crest expression of Cre recombinase directed by the proximal Pax3 promoter in transgenic mice

Ist Teil von

• Genesis (New York, N.Y. : 2000), 2000-02, Vol.26 (2), p.162-164

Ort / Verlag

New York: John Wiley & Sons, Inc

Erscheinungsjahr

2000

Quelle

MEDLINE

Beschreibungen/Notizen

• Pax3 encodes a transcription factor expressed during early to midgestation in the dorsal neural tube and somite (Goulding et al., 1991). Mutations in humans and mice indicate that Pax3 is required for neural crest and hypaxial muscle development (Auerbach, 1954; Epstein et al., 1991; Tassabehji et al., 1992). Homozygous Pax3 deficient Splotch embryos die during midgestation with cardiac and muscular defects. Cardiac abnormalities are similar to those seen in chick embryos after ablation of premigratory neural crest cells (Kirby et al., 1983). Hence, Pax3 has been implicated in migration or function of cardiac neural crest (Conway et al., 1997). Mutant embryos have other neural crest defects including absence or deficiencies of dorsal root and sympathetic ganglia. Pax3 is normally expressed in the dorsal neural tube, the region from which neural crest cells emerge, beginning at E8.5 (Goulding et al., 1991). Shortly afterward, it is expressed in presomitic mesoderm, and is later restricted to the lateral dermomyotome, which gives rise to hypaxial musculature (Tajbakhsh et al., 1997). By E14.5, expression abates, though it later re-emerges in mature Schwann cells (Kioussi et al., 1995).