Cell, 2001-06, Vol.105 (6), p.781-792
2001
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Details
- Autor(en) / Beteiligte
- Titel
- Smoothened Mutants Reveal Redundant Roles for Shh and Ihh Signaling Including Regulation of L/R Asymmetry by the Mouse Node
- Ist Teil von
- Cell, 2001-06, Vol.105 (6), p.781-792
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2001
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Genetic analyses in Drosophila have demonstrated that the multipass membrane protein Smoothened (Smo) is essential for all Hedgehog signaling. We show that Smo acts epistatic to Ptc1 to mediate Shh and Ihh signaling in the early mouse embryo. Smo and Shh/Ihh compound mutants have identical phenotypes: embryos fail to turn, arresting at somite stages with a small, linear heart tube, an open gut and cyclopia. The absence of visible left/right (L/R) asymmetry led us to examine the pathways controlling L/R situs. We present evidence consistent with a model in which Hedgehog signaling within the node is required for activation of Gdf1, and induction of left-side determinants. Further, we demonstrate an absolute requirement for Hedgehog signaling in sclerotomal development and a role in cardiac morphogenesis. [Dedicated to Rosa Beddington, a pioneer in mammalian embryology].
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0092-8674eISSN: 1097-4172DOI: 10.1016/S0092-8674(01)00385-3Titel-ID: cdi_proquest_miscellaneous_70998113
- Format
- –
- Schlagworte
- Animals, Body Patterning, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Embryo, Mammalian - embryology, Embryo, Mammalian - metabolism, Embryonic Induction, Epistasis, Genetic, Gdf1 gene, Gene Expression Regulation, Developmental, Growth Differentiation Factor 1, Growth Substances - genetics, Heart - embryology, Heart - physiology, Hedgehog protein, Hedgehog Proteins, Homeobox Protein Nkx-2.5, Homeodomain Proteins - genetics, Ihh protein, In Situ Hybridization, Intercellular Signaling Peptides and Proteins, Membrane Proteins - genetics, Mice, Models, Biological, Muscle Proteins - genetics, Mutation - genetics, Myogenic Regulatory Factor 5, Nerve Tissue Proteins - genetics, Patched Receptors, Patched-1 Receptor, Proteins - genetics, Proteins - metabolism, Ptc1 gene, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, Receptors, G-Protein-Coupled, RNA, Messenger - genetics, RNA, Messenger - metabolism, Shh protein, Signal Transduction, Smoothened protein, Smoothened Receptor, Somites - metabolism, Trans-Activators, Transcription Factors - genetics, Xenopus Proteins