The Journal of immunology (1950), 2001-07, Vol.167 (2), p.827-835
2001
Details
- Autor(en) / Beteiligte
- Titel
- CD28 Signaling Augments Elk-1-Dependent Transcription at the c-fos Gene During Antigen Stimulation
- Ist Teil von
- The Journal of immunology (1950), 2001-07, Vol.167 (2), p.827-835
- Ort / Verlag
- United States: Am Assoc Immnol
- Erscheinungsjahr
- 2001
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Untransformed CD4(+) Th1 cells stimulated with Ag and APC demonstrated a dependence on B7- and CD28-mediated costimulatory signals for the expression and function of AP-1 proteins. The induction of transactivation by the c-fos gene regulator Elk-1 mirrored this requirement for TCR and CD28 signal integration. c-Jun N-terminal kinase (JNK) (but not extracellular signal-regulated kinase or p38) protein kinase activity was similarly inhibited by neutralizing anti-B7 mAbs. Blockade of JNK protein kinase activity with SB 202190 prevented both Elk-1 transactivation and c-Fos induction. These results identify a unique role for B7 costimulatory molecules and CD28 in the activation of JNK during Ag stimulation in Th1 cells, and suggest that JNK regulates Elk-1 transactivation at the c-fos gene to promote the formation of AP-1 complexes important to IL-2 gene expression.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-1767eISSN: 1550-6606DOI: 10.4049/jimmunol.167.2.827Titel-ID: cdi_proquest_miscellaneous_70992598
- Format
- –
- Schlagworte
- Adjuvants, Immunologic - physiology, Animals, Antigen Presentation, Antigens - immunology, B7-1 Antigen - metabolism, B7-1 Antigen - physiology, c-Fos protein, CD28 antigen, CD28 Antigens - metabolism, CD28 Antigens - physiology, DNA-Binding Proteins - physiology, Elk-1 protein, Enhancer Elements, Genetic - immunology, Enzyme Activation - immunology, ets-Domain Protein Elk-1, Gene Expression Regulation - immunology, Genes, fos - immunology, Humans, Interleukin-2 - genetics, JNK Mitogen-Activated Protein Kinases, JNK protein, Jurkat Cells, Ligands, Lymphocyte Activation - immunology, MAP Kinase Kinase 1, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Mitogen-Activated Protein Kinase Kinases, Mitogen-Activated Protein Kinases - metabolism, NFATC Transcription Factors, Nuclear Proteins, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins - metabolism, Proto-Oncogene Proteins - physiology, Signal Transduction - immunology, Th1 Cells - enzymology, Th1 Cells - immunology, Th1 Cells - metabolism, Transcription Factor AP-1 - biosynthesis, Transcription Factor AP-1 - metabolism, Transcription Factor AP-1 - physiology, Transcription Factors - physiology, Transcription, Genetic - immunology, Transcriptional Activation - immunology