FEBS letters, 2000-03, Vol.470 (1), p.51-54
2000
Details
- Autor(en) / Beteiligte
- Titel
- Characterization of the interactions between the glycine transporters GLYT1 and GLYT2 and the SNARE protein syntaxin 1A
- Ist Teil von
- FEBS letters, 2000-03, Vol.470 (1), p.51-54
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2000
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In this study we have examined the effect of the SNARE protein syntaxin 1A on the glycine transporters GLYT1 and GLYT2. Our results demonstrate a functional and physical interaction between both glycine transporters and syntaxin 1A. Co-transfection of syntaxin 1A with GLYT1 or GLYT2 in COS cells resulted in approximately 40% inhibition in glycine transport. This inhibition was reversed by the syntaxin 1A-binding protein, Munc18. Furthermore, immunoprecipitation studies showed a physical interaction between syntaxin 1A and both transporters in COS cells and in rat brain tissue. Finally, we conclude that this physical interaction resulted in a partial removal of the glycine transporters from the plasma membrane as demonstrated by biotinylation studies.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(00)01297-7Titel-ID: cdi_proquest_miscellaneous_70983413
- Format
- –
- Schlagworte
- Amino Acid Transport Systems, Neutral, Animals, Antigens, Surface - genetics, Antigens, Surface - metabolism, Carrier Proteins - genetics, Carrier Proteins - metabolism, Cell Membrane - metabolism, COS Cells, Glycine Plasma Membrane Transport Proteins, Glycine transport, GLYT, glycine transporter, Membrane Proteins, Membrane trafficking, Nerve Tissue Proteins - genetics, Nerve Tissue Proteins - metabolism, Neurotransmitter reuptake, PAGE, polyacrylamide gel electrophoresis, PCR, polymerase chain reaction, Protein regulation, Rats, SNARE protein, Synaptosomal-Associated Protein 25, Syntaxin, Syntaxin 1