Details

Autor(en) / Beteiligte

• Slater-Radosti, Courtney
• Van Aller, Glenn
• Greenwood, Rebecca
• Nicholas, Richard
• Keller, Paul M.
• DeWolf, Walter E.
• Fan, Frank
• Payne, David J.
• Jaworski, Deborah D.

Titel

Biochemical and genetic characterization of the action of triclosan on Staphylococcus aureus

Ist Teil von

• Journal of antimicrobial chemotherapy, 2001-07, Vol.48 (1), p.1-6

Ort / Verlag

Oxford: Oxford University Press

Erscheinungsjahr

2001

Link zum Volltext

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Triclosan, a widely used antibacterial agent, possesses potent activity against Staphylococcus aureus. This study reports on an investigation of the antibacterial target of triclosan in this pathogen. A strain of S. aureus overexpressing the enoyl-[acyl-carrier-protein] reductase (FabI), demonstrated by Western immunoblotting, gave rise to an increase in the MIC of triclosan, while susceptibilities to a range of unrelated antibacterials were unaffected. There are approximately 12 000 molecules of FabI per cell in mid-log phase growth. This number increased by approximately three- to four-fold in the S. aureus FabI overexpressor. Triclosan selectively inhibited the incorporation of [14C]acetate into TCA-precipitable product, an indicator of fatty acid biosynthesis. Furthermore, it inhibited de novo fatty acid biosynthesis in this organism. In vitro, triclosan inhibited recombinant, purified S. aureus FabI with an IC50 of approximately 1 μM. The combination of these biochemical and genetic data provide further evidence that the mode of action of triclosan in S. aureus is via inhibition of FabI.