Details

Autor(en) / Beteiligte

• Segawa, Hiroshi
• Miyashita, Toshio
• Hirate, Yoshikazu
• Higashijima, Shin-ichi
• Chino, Naoichi
• Uyemura, Keiichi
• Kikuchi, Yutaka
• Okamoto, Hitoshi

Titel

Functional Repression of Islet-2 by Disruption of Complex with Ldb Impairs Peripheral Axonal Outgrowth in Embryonic Zebrafish

Ist Teil von

• Neuron (Cambridge, Mass.), 2001-05, Vol.30 (2), p.423-436

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2001

Quelle

MEDLINE

Beschreibungen/Notizen

• Islet-2 is a LIM/homeodomain-type transcription factor of the Islet-1 family expressed in embryonic zebrafish. Two Islet-2 molecules bind to the LIM domain binding protein (Ldb) dimers. Overexpression of the LIM domains of Islet-2 or the LIM-interacting domain of Ldb proteins prevented binding of Islet-2 to Ldb proteins in vitro and caused similar in vivo defects in positioning, peripheral axonal outgrowth, and neurotransmitter expression by the Islet-2-positive primary sensory and motor neurons as the defects induced by injection of Islet-2-specific antisense morpholino oligonucleotide. These and other experiments, i.e., mosaic analysis, coexpression of full-length Islet-2, and overexpression of the chimeric LIM domains derived from two different Islet-1 family members, demonstrated that Islet-2 regulates neuronal differentiation by forming a complex with Ldb dimers and possibly with some other Islet-2-specific cofactors.