FEBS letters, 2000-02, Vol.467 (1), p.57-60
2000
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Details
- Autor(en) / Beteiligte
- Titel
- Presence of CuZn superoxide dismutase in human serum lipoproteins
- Ist Teil von
- FEBS letters, 2000-02, Vol.467 (1), p.57-60
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2000
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- It has previously been demonstrated that CuZn-superoxide dismutase (SOD) is secreted by several human cell lines. This suggests that the circulating enzyme derives from both hemolysis and peripheral tissues as a result of cellular secretion. In the present report, we evaluated the presence of CuZn-SOD in human serum lipoproteins by both enzyme-linked immunosorbent assay and Western blot analysis of immunoprecipitated lipoprotein samples. The distribution of CuZn-SOD activity among the different lipoprotein fractions was also determined by the xanthine/xanthine oxidase method. The results demonstrated that CuZn-SOD is noticeably present in serum lipoproteins and mainly in low and high density lipoproteins (LDL and HDL). Moreover, experiments performed by incubating CuZn-SOD with a lipid emulsion and subsequent separation of the lipid fraction by ultracentrifugation showed that this enzyme associates in a saturable manner with lipids. The CuZn-SOD bound to LDL and HDL could exert a physiological protective role against oxidative damage of these lipoprotein classes that carry out a crucial role in the cholesterol transport.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(00)01123-6Titel-ID: cdi_proquest_miscellaneous_70900601
- Format
- –
- Schlagworte
- Adult, ANS, 8-anilino-1-naphthalenesulfonic acid magnesium salt, Biological Transport, Blotting, Western, BSA, bovine serum albumin, Carrier Proteins - blood, Carrier Proteins - chemistry, Carrier Proteins - classification, Carrier Proteins - metabolism, CuZn-superoxide dismutase, EDTA, ethylenediaminetetraacetic acid, Emulsions, Enzyme-Linked Immunosorbent Assay, Female, HDL, high density lipoprotein, Humans, LDL, low density lipoprotein, Lipoprotein, Lipoproteins - blood, Lipoproteins - chemistry, Lipoproteins - classification, Lipoproteins - metabolism, Lipoproteins, HDL - blood, Lipoproteins, HDL - chemistry, Lipoproteins, HDL - metabolism, Lipoproteins, LDL - blood, Lipoproteins, LDL - chemistry, Lipoproteins, LDL - metabolism, LPDS, lipoprotein deficient serum, Male, Middle Aged, Protein Binding, SOD, superoxide dismutase, Superoxide Dismutase - analysis, Superoxide Dismutase - blood, Superoxide Dismutase - metabolism, Ultracentrifugation, VLDL, very low density lipoprotein