FEBS letters, 2000-01, Vol.466 (1), p.91-95
2000
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Details
- Autor(en) / Beteiligte
- Titel
- Substrate specificity determinants of the checkpoint protein kinase Chk1
- Ist Teil von
- FEBS letters, 2000-01, Vol.466 (1), p.91-95
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2000
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The Chk1 protein kinase plays a critical role in a DNA damage checkpoint pathway conserved between fission yeast and animals. We have developed a quantitative assay for Chk1 activity, using a peptide derived from a region of Xenopus Cdc25C containing Ser-287, a known target of Chk1. Variants of this peptide were used to determine the residues involved in substrate recognition by Chk1, revealing the phosphorylation motif Φ-X-β-X-X-(S/T)*, where * indicates the phosphorylated residue, Φ is a hydrophobic residue (M>I>L>V), β is a basic residue (R>K) and X is any amino acid. This motif suggests that Chk1 is a member of a group of stress-response protein kinases which phosphorylate target proteins with related specificities.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(99)01763-9Titel-ID: cdi_proquest_miscellaneous_70883003
- Format
- –
- Schlagworte
- Amino Acid Motifs, Amino Acid Sequence, Amino Acid Substitution, Animals, Catalytic Domain - genetics, Cdc25, cdc25 Phosphatases - chemistry, cdc25 Phosphatases - genetics, cdc25 Phosphatases - metabolism, Cell Cycle, Cell cycle checkpoint, Cell Cycle Proteins - chemistry, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Checkpoint Kinase 1, Chk1, DNA Damage, Humans, In Vitro Techniques, Kinetics, Molecular Sequence Data, Peptide Fragments - chemistry, Peptide Fragments - genetics, Peptide Fragments - metabolism, Phosphorylation, Protein kinase specificity, Protein Kinases - chemistry, Protein Kinases - genetics, Protein Kinases - metabolism, Recombinant Fusion Proteins - chemistry, Recombinant Fusion Proteins - genetics, Recombinant Fusion Proteins - metabolism, Serine - chemistry, Substrate Specificity, Xenopus, Xenopus Proteins