Details

Autor(en) / Beteiligte

• ÜRMÉNYI, TURÁN P.
• BONALDO, MARIA F.
• SOARES, MARCELO B.
• RONDINELLI, EDSON

Titel

Construction of a Normalized cDNA Library for the Trypanosoma cruzi Genome Project

Ist Teil von

• The Journal of eukaryotic microbiology, 1999-09, Vol.46 (5), p.542-544

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• ABSTRACT Sequencing of the Trypanosoma cruzi genome is underway. Expressed sequence tags, obtained from cDNA libraries, facilitate mapping and gene discovery. The efficiency of large‐scale generation of such tags is increased when using normalized cDNA libraries, where the frequency of individual clones is brought within a narrow range. Repetitive sequencing of abundant clones is therefore minimized. We constructed a normalized cDNA library from epimastigotes of clone CL Brener, and the efficiency of normalization of representative clones was assessed and shown to be adequate. The normalized cDNA library has been distributed to several groups and large‐scale sequencing is currently in progress.