Toxicology letters, 2001-03, Vol.120 (1), p.43-49
2001
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Details
- Autor(en) / Beteiligte
- Titel
- Characterisation of glucuronidation and transport in V79 cells co-expressing UGT1A1 and MRP1
- Ist Teil von
- Toxicology letters, 2001-03, Vol.120 (1), p.43-49
- Ort / Verlag
- Shannon: Elsevier Ireland Ltd
- Erscheinungsjahr
- 2001
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- The co-ordinated glucuronidation and export of compounds from cells is an important determinant in the detoxification of many compounds in vivo. Many UDP-glucuronosyltransferases (UGTs) and several multidrug resistance proteins (MRPs) have been cloned and individually expressed to assess specificity of glucuronidation and transport. However, to further understand the interplay between glucuronidation and transport we are developing stable cell lines that express different combinations of UGT and MRP isoforms. V79 cells expressing both UGT1A1 and MRP1 have been established. The ability of these cell lines to both glucuronidate and transport compounds was assessed ex vivo using estradiol and bilirubin as substrates.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-4274eISSN: 1879-3169DOI: 10.1016/S0378-4274(01)00305-8Titel-ID: cdi_proquest_miscellaneous_70824474
- Format
- –
- Schlagworte
- Animals, ATP-Binding Cassette Transporters - analysis, ATP-Binding Cassette Transporters - physiology, Bilirubin - metabolism, Bilirubin glucuronide, Biological and medical sciences, Biological Transport, Cell Line, Estradiol - metabolism, Estradiol glucuronide, General aspects. Methods, Glucuronides - metabolism, Glucuronosyltransferase - analysis, Glucuronosyltransferase - physiology, Medical sciences, MRP1, Multidrug Resistance-Associated Proteins, Toxicology, UDP-glucuronosyltransferases, UGT1A1