Details

Autor(en) / Beteiligte

• Takeuchi, Osamu
• Kaufmann, Andreas
• Grote, Karsten
• Kawai, Taro
• Hoshino, Katsuaki
• Morr, Michael
• Muhlradt, Peter F
• Akira, Shizuo

Titel

Cutting Edge: Preferentially the R-Stereoisomer of the Mycoplasmal Lipopeptide Macrophage-Activating Lipopeptide-2 Activates Immune Cells Through a Toll-Like Receptor 2- and MyD88-Dependent Signaling Pathway

Ist Teil von

• The Journal of immunology (1950), 2000-01, Vol.164 (2), p.554-557

Ort / Verlag

United States: Am Assoc Immnol

Erscheinungsjahr

2000

Quelle

MEDLINE

Beschreibungen/Notizen

• Mycoplasmas and their membranes are potent activators of macrophages, the active principle being lipoproteins and lipopeptides. Two stereoisomers of the mycoplasmal lipopeptide macrophage-activating lipopeptide-2 (MALP-2) differing in the configuration of the lipid moiety were synthesized and compared in their macrophage-activating potential, the R-MALP being >100 times more active than the S-MALP in stimulating the release of cytokines, chemokines, and NO. To assess the role of the Toll-like receptor (TLR) family in mycoplasmal lipopeptide signaling, the MALP-2-mediated responses were analyzed using macrophages from wild-type, TLR2-, TLR4-, and MyD88-deficient mice. TLR2- and MyD88-deficient cells showed severely impaired cytokine productions in response to R- and S-MALP. The MALP-induced activation of intracellular signaling molecules was fully dependent on both TLR2 and MyD88. There was a strong preference for the R-MALP in the recognition by its functional receptor, TLR2.