Details

Autor(en) / Beteiligte

• Wolf, Peter
• Müllegger, Robert R.
• Peter Soyer, H.
• Hofer, Angelika
• Smolle, Josef
• Horn, Michael
• Cerroni, Lorenzo
• Hofmann-Wellenhof, Rainer
• Kerl, Helmut
• Maier, Harald
• Chadwick, Caroline A.
• Yarosh, Daniel
• Klein, Jonathan
• Bucana, Corazon
• Dunner, Kenneth
• Potten, Christopher S.
• Hönigsmann, Herbert
• Kripke, Margaret L.

Titel

Topical Treatment with Liposomes Containing T4 Endonuclease V Protects Human Skin In Vivo from Ultraviolet-Induced Upregulation of Interleukin-10 and Tumor Necrosis Factor-α

Ist Teil von

• Journal of investigative dermatology, 2000-01, Vol.114 (1), p.149-156

Ort / Verlag

Danvers, MA: Elsevier Inc

Erscheinungsjahr

2000

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Exposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-α RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.