Details

Autor(en) / Beteiligte

• Teno, Shinichi
• Kanno, Hiroko
• Oga, Satoko
• Kumakura, Shinobu
• Kanamuro, Reiko
• Iwamoto, Yasuhiko

Titel

Increased activity of membrane glycoprotein PC-1 in the fibroblasts from non-insulin-dependent diabetes mellitus patients with insulin resistance

Ist Teil von

• Diabetes research and clinical practice, 1999-08, Vol.45 (1), p.25-30

Ort / Verlag

Shannon: Elsevier Ireland Ltd

Erscheinungsjahr

1999

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Although most of patients with non-insulin-dependent diabetes mellitus (NIDDM) have insulin resistance, it is unknown whether a molecule might interfere with insulin action. Membrane glycoprotein PC-1 (plasma cell antigen-1), which inhibits insulin receptor tyrosine kinase activity, was isolated from fibroblasts of NIDDM patients. Because PC-1 content in skeletal muscle and adipose tissue correlated with whole body insulin sensitivity, PC-1 might play a role in insulin resistance. In order to know whether PC-1 activity of fibroblasts is also elevated in Japanese NIDDM patients, and whether PC-1 activity correlates with the parameters of insulin resistance in vivo or not, we measured PC-1 activity of cultured fibroblasts from 17 patients with NIDDM and seven healthy controls. PC-1 activity of the NIDDM patients was 85.2±33.1 nmol/mg per min (mean±S.D.), and was higher than that of healthy controls (42.6±12.7 nmol/mg per min, P=0.0002). Insulin sensitivity was measured in 11 of 17 NIDDM patients by the artificial pancreas. PC-1 activity of the patients with insulin resistance (glucose infusion rate <3.0 mg/kg per min, n=7) was elevated to 99.9±31.9 nmol/mg per min, while that of the other patients ( n=4) was 55.3±7.5 nmol/mg per min ( P=0.003). In conclusion, glycoprotein PC-1 activity of dermal fibroblasts is correlated with insulin resistance in patients with NIDDM.