Vaccine, 2008-06, Vol.26 (23), p.2849-2859
2008
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Details
- Autor(en) / Beteiligte
- Titel
- Modulation of immunoproteasome subunits by liposomal lipid A
- Ist Teil von
- Vaccine, 2008-06, Vol.26 (23), p.2849-2859
- Ort / Verlag
- Oxford: Elsevier Ltd
- Erscheinungsjahr
- 2008
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Liposomes are phospholipid vesicles that have been used as carriers of antigens and adjuvants. Lipid A, the endotoxic moiety of Gram-negative bacterial lipopolysaccharide is a potent adjuvant and incorporation into liposomes essentially reduces the endotoxic activity of lipid A. In this study, we analyzed the effect of liposomal lipid A [L(LA)] on the MHC class I antigen processing machinery in murine antigen presenting cells (APCs). L(LA) enhanced the surface expression of MHC class I, class II, CD80, and CD86 molecules, induced the secretion of IFN-γ, IL-12p40, TNF-α and IL-10, and caused a shift in the proteasome profile from constitutive to immunoproteasomes as observed by the induction of β2i, β5i, PA28α, and PA28β subunits. L(LA) acts through the production of IFN-γ as demonstrated with APCs generated from IFN-γ knockout mice. L(LA) therefore appears to act as an intracellular adjuvant by upregulating the antigen processing machinery, which could result in efficient antigen presentation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0264-410XeISSN: 1873-2518DOI: 10.1016/j.vaccine.2008.03.065Titel-ID: cdi_proquest_miscellaneous_70777523
- Format
- –
- Schlagworte
- Adjuvants, Immunologic - pharmacology, Allergy and Immunology, Animals, Applied microbiology, Biological and medical sciences, Blotting, Western, Bone Marrow Cells - immunology, Cell Line, Cytokines - biosynthesis, Dendritic cells, Dendritic Cells - immunology, Drug Carriers, Electrophoresis, Polyacrylamide Gel, Fatty acids, Female, Flow Cytometry, Fundamental and applied biological sciences. Psychology, Hepatitis, Immune system, Immunoproteasomes, Interferon-gamma - biosynthesis, Interferon-gamma - genetics, Interferon-gamma - physiology, Lipid A - immunology, Lipids, Liposomal lipid A, Liposomes - immunology, Lymphocytes, Macrophage Activation - immunology, Macrophages, Macrophages - immunology, Mice, Mice, Inbred C57BL, Mice, Knockout, Microbiology, Peptides, Phenotype, Proteasome Endopeptidase Complex - immunology, Studies, Toxicity, Up-Regulation - drug effects, Vaccines, Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects), Viral infections