Details

Autor(en) / Beteiligte

• Johansen, Kari
• Hinkula, Jorma
• Espinoza, Felix
• Levi, Mikael
• Zeng, Carl
• Rudén, Ulla
• Vesikari, Timo
• Estes, Mary
• Svensson, Lennart

Titel

Humoral and cell-mediated immune responses in humans to the NSP4 enterotoxin of rotavirus

Ist Teil von

• Journal of medical virology, 1999-11, Vol.59 (3), p.369-377

Ort / Verlag

New York: John Wiley & Sons, Inc

Erscheinungsjahr

1999

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Rotavirus nonstructural protein NSP4 has recently been suggested to function as a viral enterotoxin and play a role in the pathophysiological mechanism whereby rotaviruses induce diarrhea. The ability of rotavirus NSP4 to stimulate a humoral immune response was examined in naturally infected children and adults, rotavirus vaccinated children, as well as a cellular immune response in adults. In this study, 10 of 10 naturally infected children and 9 of 10 rotavirus‐vaccinated children showed a weak humoral IgG immune response to recombinant NSP4 (rNSP4) and/or a synthetic peptide corresponding to residues 114–134 of NSP4. Modest serum IgG antibody responses were detected in 20 of 20 adults. A cellular immune response to rNSP4 and/or NSP4114–134 were detected in 8 of 10 adults measured either as a T‐cell proliferative response (7 of 10), an increased production of IL‐2 (6 of 10), or increased production of interferon‐γ (8 of 10). These results indicate that NSP4 induces a humoral immune response in humans and show for the first time that NSP4 stimulates a cellular immune response, possibly including cytolytic T‐cells. J. Med. Virol. 59:369–377, 1999. © 1999 Wiley‐Liss, Inc.