Details

Autor(en) / Beteiligte

• Murphy, Anthony T.
• Witcher, Derrick R.
• Luan, Peng
• Wroblewski, Victor J.

Titel

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Ist Teil von

• Blood, 2007-08, Vol.110 (3), p.1048-1054

Ort / Verlag

Washington, DC: Elsevier Inc

Erscheinungsjahr

2007

Quelle

Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals

Beschreibungen/Notizen

• The hepatic peptide hormone hepcidin is considered the central regulator of iron metabolism. Characterizing the circulating levels of this peptide is critical to understanding its role in the development of clinically relevant syndromes, such as anemia of inflammation/chronic disease, and may provide insight into potential clinical interventions. While quantitative methods have been published for the determination of urinary hepcidin and serum prohepcidin, no definitive methods have been published for the determination of hepcidin in serum. In this report, we describe a quantitative method for the determination of both human and mouse hepcidin in serum and plasma. The method employs protein precipitation and solid-phase extraction followed by liquid chromatographic separation and tandem mass spectrometry detection. The method has a quantitative range of 0.25 ng/mL to 500 ng/mL serum for mouse hepcidin and 1 ng/mL to 500 ng/mL serum for human hepcidin. The method uses small sample volumes (50 μL for mice and 100 μL for humans) and 96-well formats for rapid sample processing. The method was used to establish baseline serum and plasma concentrations of hepcidin in normal C57Bl/6 mice and healthy human volunteers.