Details

Autor(en) / Beteiligte

• Mtchedlishvili, Zakaria
• Kapur, Jaideep

Titel

High-Affinity, Slowly Desensitizing GABAA Receptors Mediate Tonic Inhibition in Hippocampal Dentate Granule Cells

Ist Teil von

• Molecular pharmacology, 2006-02, Vol.69 (2), p.564-575

Ort / Verlag

United States: American Society for Pharmacology and Experimental Therapeutics

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• The tonic form of GABA-mediated inhibition requires the presence of slowly desensitizing GABA A receptors with high affinity, which has not yet been directly demonstrated in hippocampal neurons. Low concentration of GABA (1 μM) persistently increased baseline noise, increased membrane slope conductance, but did not affect spontaneous inhibitory postsynaptic currents (sIPSCs) in dentate granule cells (DGCs). Higher concentrations of GABA (10–100 μM) desensitized synaptic currents quickly, and there was a large residual current. Saturating concentration of GABA (1 mM) completely desensitized synaptic currents and revealed a slowly desensitizing, persistent current. Penicillin (300 μM) inhibited baseline noise without affecting mean current and inhibited decay time of sIPSCs. GABA A receptors mediating baseline noise in DGCs were sensitive to allopregnanolone, furosemide, and loreclezole and insensitive to diazepam and zolpidem. These studies demonstrate persistently open GABA A receptors on DGCs with high affinity for GABA, slow desensitization rate, and pharmacological properties similar to those of recombinant receptors containing α 4 , β 1 , and the δ subunits.