FEMS microbiology letters, 2007-08, Vol.273 (1), p.28-34
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Sesquiterpene farnesol as a competitive inhibitor of lipase activity of Staphylococcus aureus
- Ist Teil von
- FEMS microbiology letters, 2007-08, Vol.273 (1), p.28-34
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 2007
- Quelle
- Wiley-Blackwell Full Collection
- Beschreibungen/Notizen
- Abstract Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L−1) inhibits this lipase activity on a Mueller–Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophenyl palmitate (pNPP) revealed that the inhibitory action of farnesol appears to be the result of the inhibition of lipase activity rather than of its secretion into the culture medium. The inhibition was observed in all the tested 8 methicillin-susceptible S. aureus and 31 methicillin-resistant S. aureus clinical isolates. Using homogeneous lipase purified by hydrophobic interaction chromatography, it was revealed that farnesol could competitively inhibit the lipase activity against the substrate pNPP.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0378-1097eISSN: 1574-6968DOI: 10.1111/j.1574-6968.2007.00772.xTitel-ID: cdi_proquest_miscellaneous_70693044
- Format
- –
- Schlagworte
- Bacterial diseases, Bacterial sepsis, Bacteriology, Biological and medical sciences, Chromatography, Liquid, Clinical isolates, Drug resistance, Enzyme Inhibitors - pharmacology, Farnesol, Farnesol - pharmacology, Food processing industry, Fundamental and applied biological sciences. Psychology, Human bacterial diseases, Hydrophobicity, Infectious diseases, inhibitor, Lipase, Lipase - antagonists & inhibitors, Lipase - isolation & purification, Medical sciences, Metabolism. Enzymes, Methicillin, Microbiology, Minimum inhibitory concentration, p-Nitrophenyl palmitate, Palmitates - metabolism, Palmitic acid, Polysorbates - metabolism, Secretion, Staphylococcus aureus, Staphylococcus aureus - enzymology, Substrate inhibition, Substrate specificity, Substrates, Triglycerides