FEBS letters, 2001-01, Vol.488 (1), p.39-44
2001
Details
- Autor(en) / Beteiligte
- Titel
- Regulation of nuclear import by light-induced activation of caged nuclear localization signal in living cells
- Ist Teil von
- FEBS letters, 2001-01, Vol.488 (1), p.39-44
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2001
- Link zum Volltext
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- A novel fluorescence probe suitable for the study of nuclear import in living cells has been developed. The lysine-128 residue in SV40 T-antigen nuclear localization signal (NLS) was converted to a caged lysine with the amino acid blocked by a photocleavable protecting group. Following irradiation of ultraviolet (UV) light, the caged NLS conjugate translocated into and accumulated in the nucleus within 20 min similar to uncaged NLS conjugate. Maximum import rate saturated approximately 4.78±0.21% per minute when the duration of irradiation was more than 1/15 s (22 mW/cm 2). Caged NLS conjugate tended to distribute near the surface of the nucleus, and this association became stronger after UV irradiation. The caged conjugate enabled us to regulate the initial state of the reaction, both spatially and temporally.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(00)02399-1Titel-ID: cdi_proquest_miscellaneous_70635913
- Format
- –
- Schlagworte
- Active Transport, Cell Nucleus - radiation effects, Amino Acid Sequence, Animals, Antigens, Viral, Tumor - chemistry, Antigens, Viral, Tumor - genetics, BSA, bovine serum albumin, Caged nuclear localization signal, Cattle, Cell Survival, Cy7, Fluorescent Dyes - chemical synthesis, Fluorescent Dyes - chemistry, Fluorescent Dyes - metabolism, Fluorescent Dyes - radiation effects, HeLa Cells, Humans, Kinetics, Light, Light-induced activation, Lysine - chemistry, Lysine - metabolism, MBS, maleimidobenzoyl-N-hydroxysuccinimide ester, Microscopy, Fluorescence, NLS, nuclear localization signal, Nuclear import, Nuclear Localization Signals - chemical synthesis, Nuclear Localization Signals - chemistry, Nuclear Localization Signals - metabolism, Nuclear Localization Signals - radiation effects, Oligopeptides - chemical synthesis, Oligopeptides - chemistry, Oligopeptides - metabolism, Oligopeptides - radiation effects, Photolysis - radiation effects, Recombinant Fusion Proteins - metabolism, Serum Albumin - metabolism, Simian virus 40