Genomics (San Diego, Calif.), 2001-01, Vol.71 (2), p.142-149
2001
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Details
- Autor(en) / Beteiligte
- Titel
- Characterization of Human MAPRE Genes and Their Proteins
- Ist Teil von
- Genomics (San Diego, Calif.), 2001-01, Vol.71 (2), p.142-149
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 2001
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- The MAPRE genes encode the EB1 family proteins. The yeast EB1 protein had been shown to play important roles in microtubule dynamic regulation, cytokinesis, mitotic spindle positioning, and episome segregation. To facilitate functional studies of mammalian EB1 family proteins, we characterized the human MAPREgenes (MAPRE1, MAPRE2, and MAPRE3) and their proteins (EB1, RP1, and EBF3). We found that the three MAPRE genes had similar genomic structures but were on different chromosomes. We showed that EB1 family proteins appeared to be expressed ubiquitously. We identified two EBF3 proteins, which were encoded by alternatively spliced MAPRE3 mRNAs. We demonstrated that there were also two RP1 proteins, which were products of translation from different initiation codons. We showed that the three EB1 family proteins had different abilities to interact with APC in vitro, and we provided the first direct evidence for the association between endogenous EB1 and APC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0888-7543eISSN: 1089-8646DOI: 10.1006/geno.2000.6428Titel-ID: cdi_proquest_miscellaneous_70628345
- Format
- –
- Schlagworte
- Adenomatous Polyposis Coli Protein, Amino Acid Sequence, Animals, APC protein, Base Sequence, Biological and medical sciences, Chromosome Mapping, Cytoskeletal Proteins - metabolism, DNA, Complementary - isolation & purification, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, EB1 protein, EBF3 protein, Eye Proteins, Female, Fundamental and applied biological sciences. Psychology, Genes. Genome, Humans, MAPRE1 gene, MAPRE2 gene, MAPRE3 gene, Mice, Microtubule-Associated Proteins - genetics, Microtubule-Associated Proteins - metabolism, Molecular and cellular biology, Molecular genetics, Molecular Sequence Data, Multigene Family, Protein Binding, Recombinant Fusion Proteins - genetics, Recombinant Fusion Proteins - metabolism, RP1 protein, Sequence Alignment, Trans-Activators - genetics, Trans-Activators - metabolism, Transfection, Tumor Cells, Cultured