Details

Autor(en) / Beteiligte

• Kuznetsova, I.S.
• Enukashvily, N.I.
• Noniashvili, E.M.
• Shatrova, A.N.
• Aksenov, N.D.
• Zenin, V.V.
• Dyban, A.P.
• Podgornaya, O.I.

Titel

Evidence for the existence of satellite DNA-containing connection between metaphase chromosomes

Ist Teil von

• Journal of cellular biochemistry, 2007-07, Vol.101 (4), p.1046-1061

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

2007

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Physical connections between mitotic chromosomes have been reported previously. It was assumed that the interchromosome connection was based on the DNA‐protein thread. However, the data about DNA sequences and protein component in the thread is fragmentary. We demonstrated on the mouse cultured cell line and prematurely condensed chromosomes that: (a) all four mouse satellite DNA fragments (major and minor satellite, mouse satellite 3 (MS3) and mouse satellite 4 (MS4)) were involved in the thread formation; (b) MS4 was involved in the thread to the least extent among all the other fragments; (c) telomere was never a member of the thread; (d) the thread was synthesized at a late G2 phase; (e) RNA helicase p68 and CENP‐B were among the protein components of the interchromosome connection. It was shown by FACS analysis that in mouse and human cell lines: (1) the flow karyotype spectrums were never free from chromosome aggregates; (2) chromosome association did not depend on the chromosome length and each chromosome was free to associate with the other. J. Cell. Biochem. 101: 1046–1061, 2007. © 2007 Wiley‐Liss, Inc.