Experimental parasitology, 2007-08, Vol.116 (4), p.427-432
2007
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Real-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia
- Ist Teil von
- Experimental parasitology, 2007-08, Vol.116 (4), p.427-432
- Ort / Verlag
- San Diego, CA: Elsevier Inc
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We have optimized a faster and cheaper real-time PCR and developed a conventional genus specific PCR based on 18S rRNA gene to detect malaria parasites in low-grade parasitemias. Additionally, we compared these PCRs to the OptiMAL-IT™ test. Since there is no consensus on choice of standard quantitative curve in real-time assays, we decided to investigate the performance of parasite DNA from three different sources: “genome”, amplicon and plasmid. The amplicon curve showed the best efficiency in quantifying parasites. Both PCR assays detected 100% of the clinical samples tested; the sensitivity threshold was 0.5 parasite/μl and no PCR positive reaction occurred when malaria parasites were not present. Conversely, if OptiMAL-IT™ were employed for malaria diagnosis, 30% of false-negative results could be expected. We conclude that PCR assays have potential for detecting malaria parasites in asymptomatic infections, in evaluation of malaria vaccine molecule candidates, for screening blood donors, especially in endemic areas, or even in monitoring malaria therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-4894eISSN: 1090-2449DOI: 10.1016/j.exppara.2007.02.011Titel-ID: cdi_proquest_miscellaneous_70619905
- Format
- –
- Schlagworte
- 6-carboxy-fluoroscein, Animals, base pair number, Biological and medical sciences, deoxyribonucleic acid, deoxyribonucleotide triphosphates, determination coefficient, Diagnosis, DNA, DNA, Protozoan - blood, DNA, Protozoan - chemistry, dNTPs, EDTA, ethylenediaminetetraacetic acid, FAM, Fundamental and applied biological sciences. Psychology, Humans, Life cycle. Host-agent relationship. Pathogenesis, Malaria, Malaria, Falciparum - diagnosis, Malaria, Vivax - diagnosis, N,N,N,N-tetramethyl-6-carboxy-rhodamine, nanomolar, Parasitemia - diagnosis, PCR, Plasmodium falciparum - genetics, Plasmodium falciparum - isolation & purification, Plasmodium vivax - genetics, Plasmodium vivax - isolation & purification, polymerase chain reaction, Polymerase Chain Reaction - methods, Polymerase Chain Reaction - standards, Protozoa, RBC, Real-time PCR, real-time polymerase chain reaction, Recurrence, red blood cells, ribosomal ribonucleic acid, RNA, Ribosomal, 18S - genetics, rRNA, Sensitivity and Specificity, TAMRA, TBS, thick blood smears, threshold cycle, WBC, white blood cells