Details

Autor(en) / Beteiligte

• Hong, Xu
• Long, Gu
• jia, He
• Anqing, Lin
• Danzhou, Tang

Titel

Simultaneous determination of residual stilbenes and stilbene metabolites in animal tissue by liquid chromatography–tandem mass spectrometry

Ist Teil von

• Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2007-06, Vol.852 (1), p.529-533

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2007

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• A liquid chromatographic (LC)–tandem mass spectrometry (MS/MS) method was developed for simultaneous determination of stilbenes, diethylstilbestrol (DES), hexestrol (HEX), and dienoestrol (DEN) in animal tissue. Sample clean-up and analyte enrichment was performed by automated solid-phase extraction (ASPE) with a silica gel cartridge. Detection capabilities (CCβ) related to the transition products of lowest abundance for the method were 0.04–0.45 ng g −1 in tissue and were achieved using atmospheric pressure chemical ionization (APCI) in negative mode. The use of an internal standard in combination with the simplified sample preparation led to a sensitive and reliable analytical method. The recovery level of the method was 84–108% for DES and DEN between 0.5 and 5 ng g −1, and 59–87% for HEX between 0.25 and 2.5 ng g −1.