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BibTeX
Connective-tissue responses to defined biomaterial surfaces. II. Behavior of rat and mouse fibroblasts cultured on microgrooved substrates
Journal of biomedical materials research. Part A, 2008-05, Vol.85A (2), p.326-335
Grew, John C.
Ricci, John L.
Alexander, Harold
2008
Details
Autor(en) / Beteiligte
Grew, John C.
Ricci, John L.
Alexander, Harold
Titel
Connective-tissue responses to defined biomaterial surfaces. II. Behavior of rat and mouse fibroblasts cultured on microgrooved substrates
Ist Teil von
Journal of biomedical materials research. Part A, 2008-05, Vol.85A (2), p.326-335
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2008
Link zum Volltext
Quelle
Wiley Online Library - AutoHoldings Journals
Beschreibungen/Notizen
Surface microgeometry strongly influences the shapes, orientations, and growth characteristics of cultured cells, but in‐depth, quantitative studies of these effects are lacking. We investigated several contact guidance effects in cells within “dot” colonies of primary fibroblasts and in cultures of a transformed fibroblast cell line, employing titanium‐coated, microgrooved polystyrene surfaces that we designed and produced. The aspect ratios, orientations, densities, and attachment areas of rat tendon fibroblasts (RTF) colony cells, in most cases, varied (p < 0.01) by microgroove dimension. We observed profoundly altered cell morphologies, reduced attachment areas, and reduced cell densities within colonies grown on microgrooved substrates, compared with cells of colonies grown on flat, control surfaces. 3T3 fibroblasts cultured on microgrooved surfaces demonstrated similarly altered morphologies. Fluorescence microscopy revealed that microgrooves alter the distribution and assembly of cytoskeletal and attachment proteins within these cells. These findings are consistent with previous results, and taken together with the results of our in vivo and cell colony growth studies, enable us to propose a unified hypothesis of how microgrooves induce contact guidance. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Sprache
Englisch
Identifikatoren
ISSN: 1549-3296
eISSN: 1552-4965
DOI: 10.1002/jbm.a.31378
Titel-ID: cdi_proquest_miscellaneous_70452283
Format
–
Schlagworte
Animals
,
Bone Marrow Cells - cytology
,
Bone Marrow Cells - metabolism
,
Cell Culture Techniques
,
Cell Movement
,
cell-surface interactions
,
Cells, Cultured
,
Coated Materials, Biocompatible
,
Colony-Forming Units Assay
,
cytoskeleton
,
fibroblasts
,
Fibroblasts - cytology
,
Fibroblasts - metabolism
,
Implants, Experimental
,
in vitro
,
Materials Testing - methods
,
Mice
,
microgroove
,
NIH 3T3 Cells
,
Polystyrenes
,
Rats
,
Surface Properties
,
Tendons - cytology
,
Tendons - metabolism
,
Titanium
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