The Journal of parasitology, 2008-02, Vol.94 (1), p.58-67
2008
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Sequence Analysis of Ribosomal and Mitochondrial Genes of the Giant Liver Fluke Fascioloides magna (Trematoda: Fasciolidae): Intraspecific Variation and Differentiation from Fasciola hepatica
- Ist Teil von
- The Journal of parasitology, 2008-02, Vol.94 (1), p.58-67
- Ort / Verlag
- Lawrence, KS: American Society of Parasitologists
- Erscheinungsjahr
- 2008
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- Complete sequences of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna are presented. In particular, small subunit (18S) and internal transcribed spacers (ITS1 and ITS2) of the ribosomal gene (rDNA), as well as cytochrome c oxidase subunit I (cox1) and nicotinamide dehydrogenase subunit I (nad1) of the mitochondrial DNA (mtDNA), were analyzed. The 18S and ITS sequences were compared with previously published sequences of the liver fluke Fasciola hepatica. Fixed interspecific genetic differences were determined that allow molecular differentiation of F. magna and F. hepatica using either the PCR-RFLP method or PCR amplification of species-specific DNA regions. Additionally, intraspecific sequence polymorphism of the complete cox1 and nad1 mitochondrial genes in geographically distinct F. magna populations was determined. Based on the sequence divergences, short (<500 bp) variable regions suitable for broader biogeographical studies of giant liver fluke were designed.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-3395eISSN: 1937-2345DOI: 10.1645/GE-1324.1Titel-ID: cdi_proquest_miscellaneous_70448840
- Format
- –
- Schlagworte
- Adults, Animals, Annealing, Biological and medical sciences, Cattle, Cattle Diseases - parasitology, cox1 gene, Cyclooxygenase 1 - genetics, Cytochrome-c oxidase, Cytochromes, Deer, Dehydrogenases, Deoxyribonucleic acid, Design, Differentiation, Divergence, DNA, DNA, Helminth - chemistry, DNA, Intergenic - chemistry, DNA, Intergenic - genetics, DNA, Mitochondrial - chemistry, DNA, Ribosomal - chemistry, Ethanol, Fasciola hepatica, Fasciola hepatica - classification, Fasciola hepatica - genetics, Fasciolidae, Fasciolidae - classification, Fasciolidae - genetics, Fascioloides magna, Fundamental and applied biological sciences. Psychology, Gene polymorphism, General aspects, General aspects and techniques. Study of several systematic groups. Models, Genes, Genetic Variation, Genetics-Evolution, Geographic regions, internal transcribed spacers, Invertebrates, Liver, Liver - parasitology, liver flukes, Mitochondrial DNA, Molecular Sequence Data, Morphology, NAD - genetics, nad1 gene, Nemathelminthia. Plathelmintha, Nicotinamide, Nucleotide sequence, Nucleotides, Parasitology, Polymerase chain reaction, Polymerase Chain Reaction - veterinary, Polymorphism, Polymorphism, Restriction Fragment Length, Restriction fragment length polymorphism, Ribosomal DNA, RNA, Ribosomal, 18S - genetics, Sequence analysis, sequence homology, species differences, Species Specificity, Transfer RNA, Trematoda, Trematode Infections - parasitology, Trematode Infections - veterinary