Details

Autor(en) / Beteiligte

• Cattoir, Vincent
• Merabet, Lilia
• Legrand, Patrick
• Soussy, Claude-James
• Leclercq, Roland

Titel

Emergence of a Streptococcus pneumoniae isolate resistant to streptogramins by mutation in ribosomal protein L22 during pristinamycin therapy of pneumococcal pneumonia

Ist Teil von

• Journal of antimicrobial chemotherapy, 2007-05, Vol.59 (5), p.1010-1012

Ort / Verlag

Oxford: Oxford University Press

Erscheinungsjahr

2007

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Objectives The aim of this study was to characterize the mechanism of resistance to macrolides and streptogramins of a Streptococcus pneumoniae strain isolated from blood cultures in an 80-year-old patient suffering from severe pneumonia unsuccessfully treated with pristinamycin. Methods Resistance genes erm(B) and mef(A) were searched for by PCR. Portions of genes for domains V and II of the 23S rRNA (rrl) and genes for ribosomal proteins L4 (rplD) and L22 (rplV) were amplified by PCR from total genomic DNA and sequenced. Results Resistance genes erm(B) and mef(A) were not detected. Only mutation in the rplV gene encoding ribosomal protein L22 was detected. The strain contained a six amino acid insertion (107KRTAHI108) in the C-terminus of the ribosomal protein L22. Conclusions This is the first report of emergence of a pneumococcus resistant to streptogramins by mutation in ribosomal protein L22 during treatment with pristinamycin.