Details

Autor(en) / Beteiligte

• High, Katherine A
• Mingozzi, Federico
• Maus, Marcela V
• Hui, Daniel J
• Sabatino, Denise E
• Murphy, Samuel L
• Rasko, John E J
• Ragni, Margaret V
• Manno, Catherine S
• Sommer, Jurg
• Jiang, Haiyan
• Pierce, Glenn F
• Ertl, Hildegund C J

Titel

CD8 + T-cell responses to adeno-associated virus capsid in humans

Ist Teil von

• Nature medicine, 2007-04, Vol.13 (4), p.419-422

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• Hepatic adeno-associated virus (AAV)-serotype 2 mediatedgene transfer results in transgene product expression that is sustained in experimental animals but not in human subjects. We hypothesize that this is caused by rejection of transduced hepatocytes by AAV capsid-specific memory CD8+ T cells reactivated by AAV vectors. Here we show that healthy subjects carry AAV capsid-specific CD8+ T cells and that AAV-mediated gene transfer results in their expansion. No such expansion occurs in mice after AAV-mediated gene transfer. In addition, we show that AAV-2 induced human T cells proliferate upon exposure to alternate AAV serotypes, indicating that other serotypes are unlikely to evade capsid-specific immune responses.