Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...

Details

Autor(en) / Beteiligte
Titel
Polymorphisms in the methylenetetrahydrofolate reductase gene are determinant for vascular complications after liver transplantation
Ist Teil von
  • European journal of clinical nutrition, 2008-03, Vol.62 (3), p.430-435
Ort / Verlag
Basingstoke: Nature Publishing
Erscheinungsjahr
2008
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
  • Objective: The aim of this study was to evaluate the role of the C677T-MTHFR (methylenetetrahydrofolate reductase)-polymorphism (CC, CT and TT) for vascular complications in liver transplant recipients. Design: Retrospective study. Setting: Hepatology-Transplantation-Unit, Johann Wolfgang Goethe-University, Frankfurt am Main. Subjects: 48 liver transplant recipients were included, no dropouts. Methods: MTHFR polymorphism was detected by PCR amplification and digestion with Hinfl restriction enzyme. Vascular complications after liver transplantation were detected from the patients' records. The total serum homocysteine (HCY) was analyzed with high-pressure liquid chromatography. Results: In the wild-type group (CC), the HCY levels were slightly high (14.01 M). Among the patients with the CT polymorphism, the HCY values were elevated (22.53 M). In the homozygous TT group, there was a significant increase (31.26 M, P<0.01) of the HCY values. The percentage of vascular complications was higher in the heterozygous CT (47%) and homozygous TT (62.5%) group compared with wild-type CC (21%). Patients with a homozygous TT genotype of the MTHFR polymorphism with a vascular complication had a highly significant elevated HCY level compared to the other genotype groups, both with and without any vascular complications (P<0.001). Recipients with an elevated HCY and the TT polymorphism have a higher probability of developing a vascular complication after transplantation (odds ratio: 4.3 and 11.0; 95% confidence interval: 1.15, 12.25 and 1.41, 85.24). Conclusions: The C677T polymorphism in the MTHFR gene and subsequent elevation of the total serum HCY is significantly associated with an increased incidence of vascular complications in liver transplant recipients. Sponsorship: None.
Sprache
Englisch
Identifikatoren
ISSN: 0954-3007
eISSN: 1476-5640
DOI: 10.1038/sj.ejcn.1602699
Titel-ID: cdi_proquest_miscellaneous_70364464
Format
Schlagworte
amino acid metabolism, Biliary tract, Bioinformatics, Biological and medical sciences, Biology, Blood vessel, C677T-MTHFR polymorphism, Chromatography, High Pressure Liquid, Complication, Complications, Confidence intervals, Feeding. Feeding behavior, Female, Fundamental and applied biological sciences. Psychology, Gene, Gene polymorphism, Genes, Genetic aspects, genetic polymorphism, Genetic polymorphisms, Genetic Predisposition to Disease, Genotype, Health aspects, High performance liquid chromatography, Homocysteine, Homocysteine - blood, Humans, hyperhomocysteinemia, Kidneys, Liquid chromatography, Liver, liver transplant, Liver transplantation, Liver Transplantation - adverse effects, Liver Transplantation - physiology, Liver transplants, Liver, biliary tract, pancreas, portal circulation, spleen, Male, Medical sciences, Metabolic diseases, Methylenetetrahydrofolate reductase, Methylenetetrahydrofolate Reductase (NADPH2) - genetics, methylenetetrahydrofolate reductase gene, Middle Aged, Oxidoreductases, Pathology, patients, Polymerase Chain Reaction, Polymorphism, Polymorphism, Genetic, postoperative complications, Reductase, Reductases, Retrospective Studies, Risk Factors, Statistical analysis, Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases, Surgery of the digestive system, surgical procedures, operative, Transplantation, Transplants & implants, vascular diseases, Vascular Diseases - enzymology, Vascular Diseases - etiology, Vascular Diseases - genetics, Veins & arteries, Vertebrates: anatomy and physiology, studies on body, several organs or systems

Weiterführende Literatur

Empfehlungen zum selben Thema automatisch vorgeschlagen von bX