Details

Autor(en) / Beteiligte

• Yonemaru, K.(Gifu Univ. (Japan). Faculty of Applied Biological Sciences)
• Sakai, H
• Murakami, M
• Kodama, A
• Mori, T
• Yanai, T
• Maruo, K
• Masegi, T

Titel

The significance of p53 and retinoblastoma pathways in canine hemangiosarcoma

Ist Teil von

• Journal of Veterinary Medical Science, 2007, Vol.69(3), pp.271-278

Ort / Verlag

Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• To investigate whether inactivation of the p53 and retinoblastoma (Rb) protein pathways contributes to the development of canine hemangiosarcoma (HSA), we examined immunohistochemically the expression of p53, Rb, phosphorylated Rb (phospho-Rb), p16 , and cyclin D1 in 39 spontaneous canine HSAs and 10 hemangiomas. In addition, mutations in the p53 gene were analyzed by polymerase chain reaction (PCR)-single-stranded conformation polymorphism and PCR direct sequencing; furthermore, we quantified cyclin D1 mRNA by semiquantitative real-time reverse transcription-PCR. Positive immunoreactivity for p53 was observed in 17.9% of HSAs. However, mutations were not detected in these cases. The labeling indices for Rb, phospho-Rb, and cyclin D1 were markedly higher in all HSAs than in hemangiomas. Of the 7 cases with cyclin D1-positive immunoreactivity, 4 overexpressed cyclin D1 mRNA (to a level more than 10-fold higher than that of GAPDH mRNA). The pl6 protein was clearly detected in all hemangiomas; however, 82% of the neoplastic cells in HSA showed a loss of or low immunoreactivity. These results suggest that alteration of the p16-cyclin D1 -Rb pathway, rather than the p53 pathway, may be associated with the pathogenesis of canine HSA.