Details

Autor(en) / Beteiligte

• WINTER, Stuart C
• BUFFA, Francesca M
• MUSGROVE, Brian
• SLEVIN, Nick
• SLOAN, Philip
• PRICE, Pat
• WEST, Catharine M. L
• HARRIS, Adrian L
• SILVA, Priyamal
• MILLER, Crispin
• VALENTINE, Helen R
• TURLEY, Helen
• SHAH, Ketan A
• COX, Graham J
• CORBRIDGE, Rogan J
• HOMER, Jarrod J

Titel

Relation of a hypoxia metagene derived from head and neck cancer to prognosis of multiple cancers

Ist Teil von

• Cancer research (Chicago, Ill.), 2007-04, Vol.67 (7), p.3441-3449

Ort / Verlag

Philadelphia, PA: American Association for Cancer Research

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• Affymetrix U133plus2 GeneChips were used to profile 59 head and neck squamous cell cancers. A hypoxia metagene was obtained by analysis of genes whose in vivo expression clustered with the expression of 10 well-known hypoxia-regulated genes (e.g., CA9, GLUT1, and VEGF). To minimize random aggregation, strongly correlated up-regulated genes appearing in >50% of clusters defined a signature comprising 99 genes, of which 27% were previously known to be hypoxia associated. The median RNA expression of the 99 genes in the signature was an independent prognostic factor for recurrence-free survival in a publicly available head and neck cancer data set, outdoing the original intrinsic classifier. In a published breast cancer series, the hypoxia signature was a significant prognostic factor for overall survival independent of clinicopathologic risk factors and a trained profile. The work highlights the validity and potential of using data from analysis of in vitro stress pathways for deriving a biological metagene/gene signature in vivo.