Details

Autor(en) / Beteiligte

• Hu, Junjie
• Shibata, Yoko
• Voss, Christiane
• Shemesh, Tom
• Li, Zongli
• Coughlin, Margaret
• Kozlov, Michael M
• Rapoport, Tom A
• Prinz, William A

Titel

Membrane Proteins of the Endoplasmic Reticulum Induce High-Curvature Tubules

Ist Teil von

• Science (American Association for the Advancement of Science), 2008-02, Vol.319 (5867), p.1247-1250

Ort / Verlag

Washington, DC: American Association for the Advancement of Science

Erscheinungsjahr

2008

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• The tubular structure of the endoplasmic reticulum (ER) appears to be generated by integral membrane proteins, the reticulons and a protein family consisting of DP1 in mammals and Yop1p in yeast. Here, individual members of these families were found to be sufficient to generate membrane tubules. When we purified yeast Yop1p and incorporated it into proteoliposomes, narrow tubules (~15 to 17 nanometers in diameter) were generated. Tubule formation occurred with different lipids; required essentially only the central portion of the protein, including its two long hydrophobic segments; and was prevented by mutations that affected tubule formation in vivo. Tubules were also formed by reconstituted purified yeast Rtn1p. Tubules made in vitro were narrower than normal ER tubules, due to a higher concentration of tubule-inducing proteins. The shape and oligomerization of the "morphogenic" proteins could explain the formation of the tubular ER.