Details

Autor(en) / Beteiligte

• Sano, Hiroyuki
• Eguez, Lorena
• Teruel, Mary N.
• Fukuda, Mitsunori
• Chuang, Tuan D.
• Chavez, Jose A.
• Lienhard, Gustav E.
• McGraw, Timothy E.

Titel

Rab10, a Target of the AS160 Rab GAP, Is Required for Insulin-Stimulated Translocation of GLUT4 to the Adipocyte Plasma Membrane

Ist Teil von

• Cell metabolism, 2007-04, Vol.5 (4), p.293-303

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2007

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• GLUT4 trafficking to the plasma membrane of muscle and fat cells is regulated by insulin. An important component of insulin-regulated GLUT4 distribution is the Akt substrate AS160 rab GTPase-activating protein. Here we show that Rab10 functions as a downstream target of AS160 in the insulin-signaling pathway that regulates GLUT4 translocation in adipocytes. Overexpression of a mutant of Rab10 defective for GTP hydrolysis increased GLUT4 on the surface of basal adipocytes. Rab10 knockdown resulted in an attenuation of insulin-induced GLUT4 redistribution to the plasma membrane and a concomitant 2-fold decrease in GLUT4 exocytosis rate. Re-expression of a wild-type Rab10 restored normal GLUT4 translocation. The basal increase in plasma-membrane GLUT4 due to AS160 knockdown was partially blocked by knocking down Rab10 in the same cells, further indicating that Rab10 is a target of AS160 and a positive regulator of GLUT4 trafficking to the cell surface upon insulin stimulation.