Science (American Association for the Advancement of Science), 2007-03, Vol.315 (5820), p.1817-1822
2007
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Details
- Autor(en) / Beteiligte
- Titel
- Computational Design of Peptides That Target Transmembrane Helices
- Ist Teil von
- Science (American Association for the Advancement of Science), 2007-03, Vol.315 (5820), p.1817-1822
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2007
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- A variety of methods exist for the design or selection of antibodies and other proteins that recognize the water-soluble regions of proteins; however, companion methods for targeting transmembrane (TM) regions are not available. Here, we describe a method for the computational design of peptides that target TM helices in a sequence-specific manner. To illustrate the method, peptides were designed that specifically recognize the TM helices of two closely related integrins (αIIbβ₃ and αvβ₃) in micelles, bacterial membranes, and mammalian cells. These data show that sequence-specific recognition of helices in TM proteins can be achieved through optimization of the geometric complementarity of the target-host complex.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1136782Titel-ID: cdi_proquest_miscellaneous_70322180
- Format
- –
- Schlagworte
- Algorithms, Amino Acid Motifs, Amino Acid Sequence, Amino acids, Animal, plant and microbial ecology, Applied ecology, Biochemistry, Biological and medical sciences, Biophysics, Blood Platelets - physiology, Cell Membrane - chemistry, Conservation, protection and management of environment and wildlife, Databases, Protein, Dimerization, Dimers, Drug interactions, Environmental degradation: ecosystems survey and restoration, Escherichia coli - chemistry, Fluorescence Resonance Energy Transfer, Fundamental and applied biological sciences. Psychology, Humans, Integrin alphaVbeta3 - chemistry, Integrin alphaVbeta3 - metabolism, Integrins, Lipid Bilayers - chemistry, Membranes, Micelles, Models, Molecular, Molecular Sequence Data, Oncology, Optical Tweezers, Osteopontin - metabolism, Peptides, Peptides - chemistry, Peptides - metabolism, Platelet Adhesiveness, Platelet Aggregation, Platelet Glycoprotein GPIIb-IIIa Complex - chemistry, Platelet Glycoprotein GPIIb-IIIa Complex - metabolism, Platelets, Protein Engineering, Protein Structure, Secondary, Protein Structure, Tertiary, Proteins, Signal transduction, Spectrum Analysis, String theory