Details

Autor(en) / Beteiligte

• Okino, Shohei
• Suda, Masako
• Fujikura, Keitaro
• Inui, Masayuki
• Yukawa, Hideaki

Titel

Production of d-lactic acid by Corynebacterium glutamicum under oxygen deprivation

Ist Teil von

• Applied microbiology and biotechnology, 2008-03, Vol.78 (3), p.449-454

Ort / Verlag

Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag

Erscheinungsjahr

2008

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• In mineral salts medium under oxygen deprivation, Corynebacterium glutamicum exhibits high productivity of l-lactic acid accompanied with succinic and acetic acids. In taking advantage of this elevated productivity, C. glutamicum was genetically modified to produce d-lactic acid. The modification involved expression of fermentative d-lactate dehydrogenase (d-LDH)-encoding genes from Escherichia coli and Lactobacillus delbrueckii in l-lactate dehydrogenase (l-LDH)-encoding ldhA-null C. glutamicum mutants to yield strains C. glutamicum ΔldhA/pCRB201 and C. glutamicum ΔldhA/pCRB204, respectively. The productivity of C. glutamicum ΔldhA/pCRB204 was fivefold higher than that of C. glutamicum ΔldhA/pCRB201. By using C. glutamicum ΔldhA/pCRB204 cells packed to a high density in mineral salts medium, up to 1,336 mM (120 g l-¹) of d-lactic acid of greater than 99.9% optical purity was produced within 30 h.