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Reconstitution in vitro of MSP-based filopodium extension in nematode sperm
Cell motility and the cytoskeleton, 2007-04, Vol.64 (4), p.235-247
Miao, Long
Yi, Kexi
Mackey, Joy M.
Roberts, Thomas M.
2007
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Miao, Long
Yi, Kexi
Mackey, Joy M.
Roberts, Thomas M.
Titel
Reconstitution in vitro of MSP-based filopodium extension in nematode sperm
Ist Teil von
Cell motility and the cytoskeleton, 2007-04, Vol.64 (4), p.235-247
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2007
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
The major sperm protein (MSP) motility system in nematode sperm is best known for propelling the movement of mature sperm, where it has taken over the role usually played by actin in amoeboid cell motility. However, MSP filaments also drive the extension of filopodia, transient organelles composed of a core bundle of MSP filaments, that form in the late in sperm development but are not found on crawling cells. We have reconstituted filopodial extension in vitro whereby thin bundles of MSP filaments, each enveloped by a membrane sheath at their growing end, elongated at rates up to 17 μm/min. These bundles often exceeded 500 μm in length but were comprised of filaments only 1 μm long. The reconstituted filopodia assembled in the same cell‐free sperm extracts that produced MSP fibers, robust meshworks of filaments that exhibit the same organization and dynamics as the lamellipodial filament system that propels sperm movement. The filopodia and fibers that assembled in vitro both had a membranous structure at their growing end, shared four MSP accessory proteins, and responded identically to agents that alter MSP‐based motility by modulating protein phosphorylation. However, filopodia grew three‐ to four‐fold faster than fibers. The reconstitution of filopodial extension shows that, like the actin cytoskeleton, MSP filaments can adopt two architectures, bundles and meshworks, each capable of pushing against membranes to generate protrusion. The reconstitution of both forms of motility in the same in vitro system provides a promising avenue for understanding how the forces for membrane protrusion are produced. Cell Motil. Cytoskeleton 2007. © 2006 Wiley‐Liss, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 0886-1544
eISSN: 1097-0169
DOI: 10.1002/cm.20177
Titel-ID: cdi_proquest_miscellaneous_70281144
Format
–
Schlagworte
actin
,
Actins - metabolism
,
Actins - physiology
,
Animals
,
Ascaris - metabolism
,
Ascaris - physiology
,
Ascaris - ultrastructure
,
cell motility
,
cytoskeleton
,
Cytoskeleton - metabolism
,
Cytoskeleton - parasitology
,
Cytoskeleton - ultrastructure
,
Helminth Proteins - metabolism
,
Helminth Proteins - physiology
,
locomotion
,
Phosphorylation
,
Pseudopodia - metabolism
,
Pseudopodia - parasitology
,
Pseudopodia - ultrastructure
,
Sperm Motility - physiology
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