The Journal of infectious diseases, 2008-02, Vol.197 (3), p.411-419
2008
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- Autor(en) / Beteiligte
- Titel
- Dynamics of Total, Linear Nonintegrated, and Integrated HIV-1 DNA In Vivo and In Vitro
- Ist Teil von
- The Journal of infectious diseases, 2008-02, Vol.197 (3), p.411-419
- Ort / Verlag
- Chicago, IL: The University of Chicago Press
- Erscheinungsjahr
- 2008
- Quelle
- Oxford Journals 2020 Medicine
- Beschreibungen/Notizen
- Background. In patients infected with human immunodeficiency virus type 1 (HIV-1), HIV-1 DNA persists during highly active antiretroviral treatment, reflecting long-lived cellular reservoirs of HIV-1. Recent studies report an association between HIV-1 DNA levels, disease progression, and treatment outcome. However, HIV-1 DNA exists as distinct molecular forms that are not distinguished by conventional assays. Methods. We analyzed HIV-1 RNA levels in plasma, CD4 cell counts, and levels of integrated and nonintegrated HIV-1 DNA in peripheral blood mononuclear cells (PBMCs) from patients with early or chronic infection before and during antiretroviral treatment. We also studied HIV-1 DNA decay in primary CD4 T cells infected in vitro. HIV-1 DNA was analyzed using an assay that is unaffected by the location of HIV-1 integration sites. Results. HIV-1 RNA levels and total HIV-1 DNA levels decayed rapidly in patients during receipt of suppressive antiretroviral therapy. Ratios of total HIV-1 DNA levels to integrated HIV-1 DNA levels were high before initiation of therapy but diminished during therapy. Levels of linear nonintegrated HIV-1 DNA decayed rapidly in vitro (t1/ 2 = 1–4.8 days). Conclusion. Total HIV-1 DNA decays rapidly with suppression of virus replication in vivo. Clearance of HIV-1 DNA during the first 6 months of therapy reflects a disproportionate loss of nonintegrated HIV-1 DNA genomes, suggesting that levels of total HIV-1 DNA in PBMCs after prolonged virus suppression largely represent integrated HIV-1 genomes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-1899eISSN: 1537-6613DOI: 10.1086/525283Titel-ID: cdi_proquest_miscellaneous_70269340
- Format
- –
- Schlagworte
- Acquired Immunodeficiency Syndrome - drug therapy, Acquired Immunodeficiency Syndrome - epidemiology, Acquired Immunodeficiency Syndrome - genetics, Acquired Immunodeficiency Syndrome - immunology, AIDS, Antiretroviral Therapy, Highly Active, Antiretrovirals, Biological and medical sciences, California - epidemiology, CD4 Lymphocyte Count, DNA, DNA, Viral - blood, DNA, Viral - genetics, Fundamental and applied biological sciences. Psychology, Highly active antiretroviral therapy, HIV, HIV 1, HIV Infections - drug therapy, HIV Infections - epidemiology, HIV Infections - genetics, HIV Infections - immunology, HIV-1 - genetics, HIV/AIDS, Human immunodeficiency virus 1, Humans, Infections, Kinetics, Microbiology, Miscellaneous, RNA, RNA, Viral - blood, T lymphocytes, Viral Load, Virology, Virus Integration - genetics, Viruses